Mechanism of gram variability in select bacteria pdf. Principle, procedure and results february 2, 2015 acharya tankeshwar bacteriology, staining techniques in microbiology 45 gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884. The mureine structure of the bacteria wall is the basis of the color affinity. Gram staining of bacteria from environmental sources. Giemsa stain is commonly used when there is need to examine the blood smear for the parasites but is a good stain for routine examination of blood smear and used to differentiate nuclear and cytoplasmic morphology of the various cells of the blood like platelets, rbcs giemsa staining technique. As a result, variables that could cause incorrect test results may be detected and corrected. The gram stain is a classical and important staining technique that remains widely used by. Hans christian gram born in 1853 studied botany at the university of copenhagen in. Comparison of wetmount, wrightgiemsa and gramstained. Detection of acidfast bacilli afb in stained and acidwashed smears examined microscopically may provide the initial bacteriologic evidence of the presence of mycobacteria in a clinical specimen. For microbiologists the most fundamental stain was developed in 1884 by the danish bacteriologist hans christian gram.
The gram stain allows the determination of morphology, dividing bacteria into tw. The name comes from the danish bacteriologist hans christian gram, who developed the technique. Christian gram 1853 1938, qui a mis au point cette technique en 1882 et qui l a. Flood slide with crystal or gentian violet 60 seconds. In bacteriology, the gram staining allows a fast differentiation of bacteria in grampositive and gramnegative. B bbl gram slide for evaluating gram stain reagents and. Gram stain or gram staining, also called grams method, is a method of staining used to distinguish and classify bacterial species into two large groups. Gram staining is a quick procedure used to look for the presence of bacteria in tissue samples and to characterise bacteria as grampositive or gramnegative, based on the chemical and physical properties of their cell. Gram staining gram staining is most widely used differential staining in microbiology. Ziehlneelsen zn method of acid fast staining technique is used to stain mycobacterium species including m. Protocols for using the gram stains in the microbiology laboratory. The name comes from the danish bacteriologist hans christian gram, who developed the technique gram staining differentiates bacteria by the chemical and physical properties of their cell walls. Bacteria classification by gram staining free essay.